is dedicated to TargeTron (group II intron RNA) design, support, and innovation

TargeTronics, LLC


TargeTron Resources
Construction (Retargeting)
PCR Templates & Primer Mix
Gene Targeting
Frequently Asked Questions

TargeTron Vectors
LtrB TargeTron Vectors
EcI5 TargeTron Vectors

TargeTron Design
Purchase Subscription
LtrB Intron Design
EcI5 Intron Design

Free Examples
TargeTron Design

Legal Information

Group II Intron References

Popular Science

Wickelgren, I. Molecular biology. Spinning junk into gold. Science (New York, N.Y.)  300, 16469 (2003). Pubmed

Zagorski, N. Profile of Alan M. Lambowitz. Proceedings of the National Academy of Sciences of the United States of America  103, 166971 (2006). Pubmed

TargeTron Review

Lambowitz, A. M. & Zimmerly, S. Group II introns: mobile ribozymes that invade DNA. Cold Spring Harbor perspectives in biology  3, a003616 (2011). Pubmed

TargeTron Design

Perutka, J., Wang, W., Goerlitz, D. & Lambowitz, A. M. Use of computer-designed group II introns to disrupt Escherichia coli DExH/D-box protein and DNA helicase genes. Journal of molecular biology  336, 42139 (2004). Pubmed

Zhuang, F., Karberg, M., Perutka, J. & Lambowitz, A. M. EcI5, a group IIB intron with high retrohoming frequency: DNA target site recognition and use in gene targeting. RNA (New York, N.Y.)  15, 43249 (2009). Pubmed

Database for mobile group II introns

Candales, M. A. et al. Database for bacterial group II introns. Nucleic acids research  40, D18790 (2012). Pubmed The database can be accessed here

Clostridium perfringens

Chen, Y., McClane, B. A., Fisher, D. J., Rood, J. I. & Gupta, P. Construction of an alpha toxin gene knockout mutant of Clostridium perfringens type A by use of a mobile group II intron. Applied and environmental microbiology   71, 75427 (2005). Pubmed

Clostridium difficile & Clostridium botulinum

Heap, J. T., Pennington, O. J., Cartman, S. T., Carter, G. P. & Minton, N. P. The ClosTron: a universal gene knock-out system for the genus Clostridium. Journal of microbiological methods  70, 45264 (2007). Pubmed

Clostridium acetobutylicum

Shao, L. et al. Targeted gene disruption by use of a group II intron (targetron) vector in Clostridium acetobutylicum. Cell research  17, 9635 (2007). Pubmed

Clostridium phytofermentans

Tolonen, A. C., Chilaka, A. C. & Church, G. M. Targeted gene inactivation in Clostridium phytofermentans shows that cellulose degradation requires the family 9 hydrolase Cphy3367. Molecular microbiology   74, 130013 (2009). Pubmed

Staphylococcus aureus

Corvaglia, A. R. et al. A type III-like restriction endonuclease functions as a major barrier to horizontal gene transfer in clinical Staphylococcus aureus strains. Proceedings of the National Academy of Sciences of the United States of America  107, 119548 (2010). Pubmed

Yao, J. et al. Use of targetrons to disrupt essential and nonessential genes in Staphylococcus aureus reveals temperature sensitivity of Ll.LtrB group II intron splicing. RNA (New York, N.Y.)  12, 127181 (2006). Pubmed

Francisella tularensis

Rodriguez, S. A., Yu, J.-J., Davis, G., Arulanandam, B. P. & Klose, K. E. Targeted inactivation of francisella tularensis genes by group II introns. Applied and environmental microbiology  74, 261926 (2008). Pubmed

Lactococcus lactis

Frazier, C. L., San Filippo, J., Lambowitz, A. M. & Mills, D. A. Genetic manipulation of Lactococcus lactis by using targeted group II introns: generation of stable insertions without selection. Applied and environmental microbiology  69, 11218 (2003). Pubmed

Broad-Host-Range Targetron Vector

Yao, J. & Lambowitz, A. M. Gene targeting in gram-negative bacteria by use of a mobile group II intron (Targetron) expressed from a broad-host-range vector. Applied and environmental microbiology  73, 273543 (2007). Pubmed


Zhong, J., Karberg, M. & Lambowitz, A. M. Targeted and random bacterial gene disruption using a group II intron (targetron) vector containing a retrotransposition-activated selectable marker. Nucleic acids research  31, 165664 (2003). Pubmed


Mastroianni, M. et al. Group II intron-based gene targeting reactions in eukaryotes. PloS one  3, e3121 (2008). Pubmed